Abstract: This paper aims to improve the effectiveness of industry-university-research Institute cooperation from the perspective of multidimensional actor interaction. We choose Institute of Shenyang Blower and DUT as sample case, using exploratory single case study method to explore the interactive form of research-production and its mechanism of action in the process of knowledge transfer base on the model of SECI. Firstly, this paper introduces the evolution process of the cooperation between the project cooperation, the technology center and the cooperative research institute; Secondly, according to the analysis of the case, we find that there exists a four dimensional actor interaction in the process of knowledge transfer in industry-university-research institute cooperation. The four dimensional actor interactions are interest interaction, emotional interaction, task interaction and behavioral interaction. We also analyze the pattern of the four dimensional actor interactions in knowledge transfer, and then explore the mechanism of action of the four dimensional actor interaction in the process of industry-university-research institute cooperation, further clarifying that different interaction dimensions play diversified roles in different stages of knowledge transfer. Lastly, we construct an integrated model of knowledge transfer of industry-university-research institute based on multidimensional actor interaction. The model explains the general process of multidimensional interaction.