Abstract: Entrepreneurial calling, as entrepreneurs feel the strong summon from the entrepreneurial field, is an important work value orientation that drives entrepreneurs to identify with entrepreneurship from the heart, and achieve the purpose and meaning of life. Research in the field of management and organization often calls for an emphasis on calling as a work orientation, analyzing its positive effects on individual behavior, job performance, social identity, etc. The calling for research in the field of entrepreneurship is still in its infancy. Researchers have attempted to combine the calling concepts from the field of management and organization, starting from the intrinsic motivations and psychological context of entrepreneurs, to explore the mechanisms and boundary conditions of the calling driving entrepreneurial efforts, making useful attempts to expand the special application of the calling in the field of entrepreneurship. However, we still know a little about what the entrepreneurial results will the entrepreneurial calling bring, from the perspective of entrepreneurial behavior to analyze the mechanism of entrepreneurial calling on the growth of entrepreneurial enterprises, so as to enrich and deepen the latest research results of entrepreneurial calling. Based on the Career Construction Theory and Implicit Theories, we take 316 questionnaires of entrepreneurs as samples, combine with entrepreneurial persistence to analyze the mechanism of the impact of entrepreneurial calling on the growth of entrepreneurial enterprises, and test the moderating effect of growth mindset on the relationship. We found that the entrepreneurial calling not only promotes the growth of entrepreneurial enterprises, but also promotes the growth of entrepreneurial enterprises through entrepreneurial persistence. When entrepreneurs have more growth mindset, the entrepreneurial calling is more conducive to the growth of entrepreneurial enterprises. The results are still robust by changing the sample size and alternative explanations of variables. Further analysis shows that the entrepreneurial calling of opportunistic entrepreneurs and serial entrepreneurs has a stronger promoting effect on the growth of entrepreneurial enterprises, but with the increase of enterprise age, the positive impact of entrepreneurial calling on the growth of entrepreneurial enterprises is weakened. We deepen the existing research from the following aspects: Firstly, from the perspective of entrepreneurial persistence and growth mindset, analyze the impact mechanism and boundary conditions of entrepreneurial calling on the growth of entrepreneurial enterprises, and broaden the micro-perspective of entrepreneurial growth from the perspective of entrepreneurial psychology and entrepreneurial behavior. Secondly, deepen the understanding of the calling under the entrepreneurial context, explore the mechanism of the impact of the calling on entrepreneurial persistence and the growth of entrepreneurial enterprises in combination with the internal psychological context of entrepreneurs, and enrich the research literature on the results of the entrepreneurial calling. Thirdly, the Career Construction Theory in the field of management and organization is applied to explore the career construction law of entrepreneurs in the entrepreneurial context, and the regulating role of growth mindset is analyzed in combination with the Implicit Theories, and the application context and scope of these two theories are extended. Future study can adopt different methods, such as longitudinal tracking research design, to reveal the dynamic mechanism of entrepreneurial calling affecting the growth of entrepreneurial enterprises. In addition, more objective situational factors of entrepreneurial calling driving the growth of entrepreneurial enterprises are explored to increase the scope of application of research conclusions.